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OceanOpticsSpectrometer>> Fluorescence Spectrometer>>USB4000-FL Fluorescence Spectrometer  

 
USB4000-FL Fluorescence Spectrometer

Our next-generation miniature spectrometer platform for fluorescence spectroscopy is the USB4000-FL. Preconfigured with an L4 Detector Collection Lens to increase light-collection efficiency and reduce stray light, the high-sensitivity USB4000-FL covers the 360-1000 nm wavelength range. It also features a programmable microcontroller that lets you implement operating parameters in the software -- control the spectrometer and external excitation sources, and even retrieve data on external objects!

Programmable Microcontroller

The USB4000-FL's onboard programmable microcontroller provides you with considerable flexibility in controlling the spectrometer and accessories. Through a 22-pin connector, you can implement all operating parameters in the software: control light sources, create processes, and retrieve information on external objects. You have access to 8 user-programmable digital Inputs/Outputs for interfacing to other equipment. (Contact an Applications Scientist regarding what you'll need to program the GPIOs.)

Optimal Performance

The USB4000-FL provides you with an affordable, high-performance 3648-element linear CCD-array detector with an optical bench that's small enough to fit into the palm of your hand. The USB4000-FL works the same way as other Ocean Optics spectrometers; it accepts light energy transmitted through single-strand optical fiber and disperses it via a fixed grating across the linear CCD array detector, which is responsive from 200-1100 nm.

Plug and Play Operation

The USB4000-FL interfaces to a PC, PLC or other embedded controllers via USB 2.0 or RS-232 serial port. Data unique to each spectrometer is programmed into a memory chip on the USB4000-FL; our spectrometer operating software reads these values for easy setup and hot swapping among PCs.

Accessory Filters

The USB4000-FL can be used with our LVF-series Linear Variable Filters to construct systems with excellent separation of excitation and fluorescence energy. Use our LVF-L Linear Variable Low-pass Filter to fine tune your excitation source for maximum signal with minimum overlap; and/or use our LVF-H Linear Variable High-pass Filter on the detection side. Inline holders and cuvette fixtures make deployment of these filters easy.

Specifications

Physical

Spectrometer Dimensions: 89.1 mm x 63.3 mm x 34.4 mm
Weight: 190 grams
Detector Specifications

Detector:

Toshiba TCD1304AP Linear CCD array

Detector range: 200-1100 nm

Pixels:

3648 pixels

Pixel size:

8 μm x 200 μm

Pixel well depth:

100,000 electrons

Signal-to-noise ratio: 300:1 (at full signal)
A/D resolution: 16 bit
Dark noise: 50 RMS counts
Corrected linearity: < 0.2%
Sensitivity: 130 photons/count at 400 nm; 60 photons/count at 600 nm
Optical Bench
Design: f/4, Asymmetrical crossed Czerny-Turner
Focal length: 42 mm input; 68 mm output
Entrance aperture: 200 µm wide slit
Grating: Grating #3 -- groove density 600 lines per millimeter, 360-1000 nm, 500 nm blaze
Detector collection lens: Yes, L4
Fiber optic connector: SMA 905 to 0.22 numerical aperture single-strand optical fiber
Spectroscopic
Wavelength range: 360-1000 nm
Optical resolution: ~10.0 nm FWHM
Signal-to-noise ratio: 300:1 (at full signal)
A/D resolution: 16 bit
Dark noise: 50 RMS counts
Integration time: 3.8 ms - 10 seconds
Dynamic range: 2 x 108 (system), 1300:1 for a single acquisition
Stray light: <0.05% at 600 nm; 0.10% at 435 nm
Electronics
Power consumption: 250 mA @ 5 VDC
Data transfer speed: Full scans to memory every 5 ms with USB 2.0 port
Inputs/Outputs: Yes, 8 onboard digital user-programmable GPIOs
Analog channels: No
Auto nulling: Yes
Trigger modes: 4 modes
Strobe functions: Yes
Connector: 22-pin connector
Computer
Operating systems: Windows 98/Me/2000/XP, Mac OS X and Linux with USB port; Any 32-bit Windows OS with serial port
Computer interfaces: USB 2.0 @ 480 Mbps (USB1.1 compatible); RS-232 (2-wire) @ 115.2 K baud
Peripheral interfaces: SPI (3-wire); I2C inter-integrated circuit
 
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